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Title GFP whole cell microbial biosensors : scale-up and scale-down effects on biopharmaceutical processes / Frank Delvigne [and others].

Publication Info. New York, N.Y. : ASME ; New York, N.Y. (222 East 46th Street, New York, NY 10017) : Momentum Press, 2013.

Item Status

Description 1 online resource (1 online resource (43 pages)) : illustrations, digital file.
Physical Medium polychrome
Description text file
Series Biomedical & nanomedical technologies
Biomedical & nanomedical technologies.
Note Title from PDF title page (viewed on January 26, 2013).
Bibliography Includes bibliographical references (pages 36-43).
Contents 1. Interaction between fluid flow and microbial cells: importance of the operating scale -- 1.1 Different scales involved in the development of microbial bioprocesses -- 1.2 Hydrodynamic modeling of the substrate heterogeneites perceived by cells.
2. Stochastic simulation of the displacement of microbial cells along concentration field -- 2.1 Microbial stress response: different timescales involved.
3. Experimental results gained from the physiological response of GFP biosensors in scale-down conditions -- 3.1 General stress response in scale-down bioreactors: rpoS::GFP -- 3.2 Use of destabilized GFP variants.
4. Another source of information: protein leakage and the study of the secretome -- References.
Summary Two strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical engineering improvement of the bioreactors and scaling-up rules. This work is more particularly dedicated to this second class of parameters.
Local Note eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America
Subject Microbial biotechnology.
Microbial biotechnology.
Green fluorescent protein.
Green fluorescent protein.
Biosensors.
Biosensors.
Indexed Term biomedical and nanomedical technologies
engineering
microbial cells
Genre/Form Electronic books.
Electronic books.
Added Author Delvigne, Frank.
Added Title Green fluorescent protein whole cell microbial biosensors
Scale-up and scale-down effects on biopharmaceutical processes
Other Form: Print version: 9781606504277
ISBN 9781606504178 (electronic book)
1606504177 (electronic book)
1606504274
9781606504277
9781606504277 (print)