LEADER 00000cam a2200493Ma 4500 001 on1225543988 003 OCoLC 005 20211008041809.0 006 m o d 007 cr cnu---unuuu 008 201205s2020 xx o 000 0 eng d 020 1839162864 020 9781839162862|q(electronic bk.) 035 (OCoLC)1225543988 040 EBLCP|beng|cEBLCP|dN$T|dEBLCP|dN$T|dOCLCO|dUKAHL|dOCLCF 049 RIDW 050 4 TJ853.4.M53 082 04 620.1/06|223 090 TJ853.4.M53 100 1 Ren, Carolyn. 245 10 Droplet Microfluidics|h[electronic resource]. 260 Cambridge :|bRoyal Society of Chemistry,|c2020. 300 1 online resource (323 p.). 490 1 ISSN 500 Description based upon print version of record. 500 5.2.3 Examples of Data and Conclusions. 500 Edited by two leaders, this book has drawn together expertise from around the globe to form a unified, cohesive resource for the droplet microfluidics community. 505 0 Intro -- Halftitle -- Series Title -- Title -- Copyright - - Preface -- Contents -- Chapter 1 History and Current Status of Droplet Microfluidics 1 -- 1.1 Introduction -- 1.1.1 Single-phase Microfluidics -- 1.1.2 Challenges of Single-phase Microfluidics -- 1.1.3 Emergence of Droplet Microfluidics -- 1.2 Challenges and Opportunities of Droplet Microfluidics and Motivation of this Book -- 1.2.1 Interdisciplinary Nature -- 1.2.2 Technological Improvements Towards an Enabling Tool -- 1.3 Summary -- References -- Chapter 2 Fundamentals 15 -- 2.1 Introduction -- 2.2 Basic Concepts of Capillarity 505 8 2.2.1 Interface and Contact Angle -- 2.2.2 Interfacial (and Surface) Tension -- 2.2.3 Young's Equation, Wettability and Wetting -- 2.2.4 Young-Laplace Equation and Capillary Pressure -- 2.3 Dimensionless Numbers in Microfluidics -- 2.3.1 Dimensionless Numbers of Forces -- 2.3.2 Dimensionless Numbers of Mass Transfer -- 2.3.3 Other Dimensionless Numbers -- 2.4 Droplet Generation in Microfluidics -- 2.4.1 Common Geometrical Generators -- 2.4.2 External Forces Based Active Methods -- 2.5 Size Scaling of Generated Droplets -- 2.6 Hydrodynamics of Taylor-type Droplets in Microchannels 505 8 2.6.1 Thin Films in Capillaries and Square Microchannels - - 2.6.2 Pressure Drops of Droplets Flowing in Microchannels -- 2.6.3 Moving Speeds of Droplets -- 2.6.4 Flow Fields Within Droplets -- 2.7 Conclusion -- References -- Chapter 3 Technological Development -- Droplet as a Tool 45 -- 3.1 Introduction -- 3.2 Active Droplet Generation -- 3.2.1 Electrical Control -- 3.2.2 Magnetic Control -- 3.2.3 Thermal Control -- 3.2.4 Pneumatic Control -- 3.2.5 Piezoelectric Control -- 3.3 Active Droplet Sorting -- 3.3.1 Electrical Control -- 3.3.2 Magnetic Control -- 3.3.3 Thermal Control 505 8 3.3.4 Pneumatic Control -- 3.3.5 Acoustic Control -- 3.4 Active Droplet Coalescence -- 3.4.1 Electric Control -- 3.4.2 Magnetic Control -- 3.4.3 Thermal Control -- 3.4.4 Pneumatic Control -- 3.4.5 Acoustic Control -- 3.4.6 Optical Control -- 3.5 Conclusion -- References -- Chapter 4 Droplet Gene Analysis -- Digital PCR 89 -- 4.1 Introduction -- 4.2 Droplet Digital PCR Workflow -- 4.3 Fluorophores for ddPCR Applications -- 4.4 Surfactants -- Droplet Stabilizing Agents -- 4.5 Molecular Adsorption/ retention -- 4.6 Template Preparation -- 4.7 Multiplex Assays 505 8 4.8 Commercial Droplet Digital PCR Systems -- 4.8.1 Bio- Rad QX200 -- 4.8.2 RainDrop Plus -- 4.8.3 Stilla Technologies' Naica -- 4.8.4 Comparison of Commercial Systems -- 4.9 Droplet Digital PCR Applications -- 4.10 Emerging Alternative Techniques -- References -- Chapter 5 Microfluidic Applications in Single-cell Genomic, Transcriptomic and Proteomic Analysis 122 -- 5.1 Introduction -- 5.1.1 Single-cell Analysis in Biology -- 5.1.2 Single-cell Analysis in Cancer -- 5.1.3 Microfluidics Overview -- 5.2 Genomic DNA Applications: SNV and CNV Detection -- 5.2.1 Overview -- 5.2.2 Workflow 590 eBooks on EBSCOhost|bEBSCO eBook Subscription Academic Collection - North America 650 0 Microdroplets. 650 7 Microdroplets.|2fast|0(OCoLC)fst01894575 655 0 Electronic books. 655 4 Electronic books. 700 1 Lee, Abraham. 776 08 |iPrint version:|aRen, Carolyn|tDroplet Microfluidics |dCambridge : Royal Society of Chemistry,c2020 |z9781788017695 830 0 ISSN. 856 40 |uhttps://rider.idm.oclc.org/login?url=https:// search.ebscohost.com/login.aspx?direct=true&scope=site& db=nlebk&AN=2694779|zOnline ebook via EBSCO. Access restricted to current Rider University students, faculty, and staff. 856 42 |3Instructions for reading/downloading the EBSCO version of this ebook|u http://guides.rider.edu/ebooks/ebsco 948 |d20211213|cEBSCO|tEBSCOebooksacademic NEW Oct-Nov 5018 |lridw 994 92|bRID