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BestsellerE-book
Author Lutz, Norbert.

Title Methodologies for Metabolomics : Experimental Strategies and Techniques.

Imprint Cambridge : Cambridge University Press, 2012.

Item Status

Description 1 online resource (642 pages)
Contents Contributors; Section 1 Basic Methodological Strategies in Metabolomic Research; 1 Exploring the Human Metabolome by Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry; 1. Brief History of Human Metabolomics; 2. Sample Preparation for Human Metabolomic Studies; 3. Human Metabolite Identification by Nuclear Magnetic Resonance Spectroscopy; 4. Human Metabolite Identification by Gas Chromatography-Mass Spectrometry; 5. Human Metabolite Identification by Liquid Chromatography-Mass Spectrometry; 6. Comparison of Technologies; 7. Conclusion; Acknowledgments.
2 Methodological Requirements for Lipidomics Research1. Sample Preparation and Lipid Extraction; 2. Sample Preparation; 3. Lipid Extraction; 4. Sample Preparation and Lipid Extraction of Subcellular Organelles; 5. Chromatography-Coupled Mass Spectrometry of Lipids; 6. Shotgun Lipidomics -- Direct Infusion-Based Mass Spectrometry of Lipids; 7. Postacquisition Data Processing and Bioinformatics; 8. Conclusion; Acknowledgments; 3 Biological Methods for Metabolic Research; 1. Gene Overexpression as a Tool for Metabolomic Studies; 1.1. Gene Transfection: Plasmid Vectors.
1.2. Gene Transduction: Viral Vectors2. Gene Silencing as a Tool for Metabolomic Studies; 3. Validation of Gene Overexpression or Silencing by mRNA; 3.1. Gene Expression Analysis; 3.1.1. Reverse Transcriptase Polymerase Chain Reaction; 3.1.2. Real-Time Polymerase Chain Reaction; 4. Validation of Gene Overexpression or Silencing by Protein Level Analysis and Enzyme Assays; 4.1. Western Blotting; 4.1.1. Blotting; 4.1.2. Immunodetection; 4.2. Enzyme Assays; 4.2.1. Protocols for Analysis of Choline Kinase- Activity; 4.2.2. Protocol for Analysis of Phospholipase D Activity.
5. Enzymatic Assays as a Tool of Phospholipid Metabolism Analysis: Effects of Choline Kinase- Inhibition on the Balance of Phospholipid Metabolites5.1. Protocol for Lipid Analysis; 5.2. Protocol for Analysis of Phosphatidylinositol-3-Kinase Activity; 6. Phospholipid Profile Analysis by Mass Spectrometry; 6.1. Phospholipid Extraction; 6.2. Analytical Technique for Phospholipids: Mass Spectrometry; Section 2 Metabolomic Mass Spectrometry: Experimental Techniques and Bioinformatics.
4 Considerations in Sample Preparation, Collection, and Extraction Approaches Applied in Microbial, Plant, and Mammalian Metabolic Profiling1. Considerations in the Culturing, Collection, Preparation, and Extraction of Microbial Samples; 1.1. Sample Types; 1.2. Culture Methods; 1.2.1. Batch Growth; 1.2.2. Continuous Culture; 1.2.3. Selection of Media; 1.3. Experimental Considerations in the Collection and Preparation of Samples; 1.3.1. Quenching of Cultures; 1.3.2. Assessment of Leakage; 1.3.3. Extraction of Intracellular Metabolites.
Note 2. Considerations in the Growth, Harvest, Preparation, and Extraction of Plant Materials.
Summary Methodologies for Metabolomics provides comprehensive descriptions of the newest methodological strategies and techniques in metabolomic research.
Bibliography Includes bibliographical references and index.
Language English.
Local Note eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America
Subject Mass spectrometry -- Methods.
Metabolism -- Disorders -- Diagnosis.
Metabolites.
Genre/Form Electronic books.
Methods (Music)
Added Author Sweedler, Jonathan.
Wevers, Ron.
Other Form: Print version: Lutz, Norbert. Methodologies for Metabolomics : Experimental Strategies and Techniques. Cambridge : Cambridge University Press, ©2012 9780521765909
ISBN 9781139615334
1139615335
9781283870375 (MyiLibrary)
1283870371 (MyiLibrary)
9780511996634 (ebook)
0511996632 (ebook)
9780521765909 (hardback)
0521765900 (hardback)
9781139624633 (electronic bk.)
1139624636 (electronic bk.)