| Description |
1 online resource (xxii, 344 pages) : illustrations (some color) |
| Physical Medium |
polychrome |
| Description |
text file |
| Summary |
Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references. |
| Contents |
Foreword. Biology of the Filamentous Bacteriophage. Principles and Applications of Phage Display. Vectros for Phage Display. Construction of Random Peptide Libraries in Bacteriophage. Construction and Screening of Antibody Display Libraries. Phagemeid-Displayed Peptide Libraries. Phage Libraries Displaying Random Peptides. Derived Form a Target Sequence. Display and Selection of Proteins on Genetic Packages. A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand Interactions Made Possible By Linkage of Recogntion and Replication Functions. Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design. Use of Filamentous Virus Particles as Immunogens. Screening Phage Displayed Random Peptide Libraries. Phage Display Factors Affecting Planning Efficieny. Preparation of Second Generation Phage Libraries. Nonradioactive Sequencing of Random Peptide Recombinant Phage. Measurement of Peptide Binding Affinities Using Fluorescence Polarization. |
| Bibliography |
Includes bibliographical references and index. |
| Access |
Use copy Restrictions unspecified MiAaHDL |
| Reproduction |
Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL |
| System Details |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
| Processing Action |
digitized 2010 HathiTrust Digital Library committed to preserve MiAaHDL |
| Local Note |
eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America |
| Subject |
Bacteriophages -- Laboratory manuals.
|
|
Bacteriophages. |
| Genre/Form |
Laboratory manuals.
|
| Subject |
Microbial biotechnology -- Laboratory manuals.
|
|
Microbial biotechnology. |
|
Viral proteins -- Laboratory manuals.
|
|
Viral proteins. |
|
Affinity chromatography -- Laboratory manuals.
|
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Affinity chromatography. |
|
Peptides -- Biotechnology -- Laboratory manuals.
|
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Peptides -- Biotechnology. |
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Proteins -- Biotechnology -- Laboratory manuals.
|
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Proteins -- Biotechnology. |
|
Bacteriophages. |
|
Peptides. |
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Proteins. |
|
Chromatography, Affinity. |
| Indexed Term |
Microbiology Laboratory techniques |
| Genre/Form |
Laboratory Manual.
|
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Electronic books.
|
|
Laboratory manuals.
|
| Added Author |
Kay, Brian K.
|
|
Winter, Jill.
|
|
McCafferty, John, Dr.
|
| Other Form: |
Print version: Phage display of peptides and proteins. San Diego : Academic Press, 1996 0124023800 9780124023802 (DLC) 96013576 (OCoLC)34409484 |
| ISBN |
9780124023802 |
|
0124023800 |
|
9780080538709 (electronic book) |
|
0080538703 (electronic book) |
|
