LEADER 00000cam a2200769Ia 4500 001 ocn162129287 003 OCoLC 005 20160527040706.7 006 m o d 007 cr cn||||||||| 008 070802s2000 caua obf 001 0 eng d 019 180757165|a182732302|a647695710|a823828618|a823898354 |a824089646|a824136187 020 9780122093708 020 0122093704 020 9781435608092|q(electronic book) 020 1435608097|q(electronic book) 020 0080536905 020 9780080536903 020 128103276X 020 9781281032768 035 (OCoLC)162129287|z(OCoLC)180757165|z(OCoLC)182732302 |z(OCoLC)647695710|z(OCoLC)823828618|z(OCoLC)823898354 |z(OCoLC)824089646|z(OCoLC)824136187 037 88709:88709|bElsevier Science & Technology|nhttp:// www.sciencedirect.com 040 OPELS|beng|epn|cOPELS|dOCLCQ|dN$T|dYDXCP|dIDEBK|dE7B|dNRU |dOCLCQ|dOCLCO|dOCLCQ|dOCLCF|dOCL|dOCLCQ 049 RIDW 050 4 QP98|b.D46 2000eb 072 7 MED|x075000|2bisacsh 072 7 SCI|x036000|2bisacsh 072 7 MJCM|2bicssc 082 04 612.1/1825|222 090 QP98|b.D46 2000eb 100 1 Denomme, Gregory A.|0https://id.loc.gov/authorities/names/ n00122912 245 10 Molecular protocols in transfusion medicine /|cGregory A. Denomme, Maria Rios, Marion E. Reid. 264 1 San Diego, CA :|bAcademic Press,|c2000. 300 1 online resource (x, 186 pages) :|billustrations 336 text|btxt|2rdacontent 337 computer|bc|2rdamedia 338 online resource|bcr|2rdacarrier 340 |gpolychrome|2rdacc 347 text file|2rdaft 504 Includes bibliographical references and index. 505 0 Front Cover; Molecular Protocols in Transfusion Medicine; Copyright Page; Contents; Preface; List of Abbreviations; Part 1: Introduction; Part 2: General Considerations; Part 3: Protocols; Part 4: Red Cell Blood Groups; Part 5: Platelet Blood Groups; Part 6: Neutrophil Blood Groups; Part 7: Appendices; Index; Useful Tables. 520 This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests). 588 0 Print version record. 590 eBooks on EBSCOhost|bEBSCO eBook Subscription Academic Collection - North America 650 0 Blood groups|0https://id.loc.gov/authorities/subjects/ sh85014985|vHandbooks, manuals, etc.|0https://id.loc.gov/ authorities/subjects/sh99001300 650 0 Genetic polymorphisms|0https://id.loc.gov/authorities/ subjects/sh85053865|vHandbooks, manuals, etc.|0https:// id.loc.gov/authorities/subjects/sh99001300 650 0 Blood|xTransfusion|0https://id.loc.gov/authorities/ subjects/sh85014949|vHandbooks, manuals, etc.|0https:// id.loc.gov/authorities/subjects/sh99001300 650 7 Blood groups.|2fast|0https://id.worldcat.org/fast/834737 650 7 Genetic polymorphisms.|2fast|0https://id.worldcat.org/fast /940074 650 7 Blood|xTransfusion.|2fast|0https://id.worldcat.org/fast/ 834613 650 12 Blood Grouping and Crossmatching.|0https://id.nlm.nih.gov/ mesh/D001788 650 22 Blood Transfusion.|0https://id.nlm.nih.gov/mesh/D001803 650 22 Polymorphism, Genetic.|0https://id.nlm.nih.gov/mesh/ D011110 655 2 Laboratory Manual.|0https://id.nlm.nih.gov/mesh/D020484 655 4 Electronic books. 655 7 Handbooks and manuals.|2fast|0https://id.worldcat.org/fast /1423877 655 7 Handbooks and manuals.|2lcgft|0https://id.loc.gov/ authorities/genreForms/gf2014026109 700 1 Rios, Maria.|0https://id.loc.gov/authorities/names/ n00122914 700 1 Reid, Marion E.|0https://id.loc.gov/authorities/names/ n96802827 776 08 |iPrint version:|aDenomme, Gregory A.|tMolecular protocols in transfusion medicine.|dSan Diego, CA : Academic Press, 2000|z0122093704|z9780122093708|w(DLC) 99068336 |w(OCoLC)44184819 856 40 |uhttps://rider.idm.oclc.org/login?url=http:// search.ebscohost.com/login.aspx?direct=true&scope=site& db=nlebk&AN=141468|zOnline eBook. Access restricted to current Rider University students, faculty, and staff. 856 42 |3Instructions for reading/downloading this eBook|uhttp:// guides.rider.edu/ebooks/ebsco 901 MARCIVE 20231220 948 |d20160615|cEBSCO|tebscoebooksacademic|lridw 994 92|bRID