| Description |
1 online resource (323 pages). |
| Physical Medium |
polychrome |
| Description |
text file |
| Series |
ISSN
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ISSN.
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| Note |
Description based upon print version of record. |
| Contents |
Intro -- Halftitle -- Series Title -- Title -- Copyright -- Preface -- Contents -- Chapter 1 History and Current Status of Droplet Microfluidics 1 -- 1.1 Introduction -- 1.1.1 Single-phase Microfluidics -- 1.1.2 Challenges of Single-phase Microfluidics -- 1.1.3 Emergence of Droplet Microfluidics -- 1.2 Challenges and Opportunities of Droplet Microfluidics and Motivation of this Book -- 1.2.1 Interdisciplinary Nature -- 1.2.2 Technological Improvements Towards an Enabling Tool -- 1.3 Summary -- References -- Chapter 2 Fundamentals 15 -- 2.1 Introduction -- 2.2 Basic Concepts of Capillarity |
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2.2.1 Interface and Contact Angle -- 2.2.2 Interfacial (and Surface) Tension -- 2.2.3 Young's Equation, Wettability and Wetting -- 2.2.4 Young-Laplace Equation and Capillary Pressure -- 2.3 Dimensionless Numbers in Microfluidics -- 2.3.1 Dimensionless Numbers of Forces -- 2.3.2 Dimensionless Numbers of Mass Transfer -- 2.3.3 Other Dimensionless Numbers -- 2.4 Droplet Generation in Microfluidics -- 2.4.1 Common Geometrical Generators -- 2.4.2 External Forces Based Active Methods -- 2.5 Size Scaling of Generated Droplets -- 2.6 Hydrodynamics of Taylor-type Droplets in Microchannels |
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2.6.1 Thin Films in Capillaries and Square Microchannels -- 2.6.2 Pressure Drops of Droplets Flowing in Microchannels -- 2.6.3 Moving Speeds of Droplets -- 2.6.4 Flow Fields Within Droplets -- 2.7 Conclusion -- References -- Chapter 3 Technological Development -- Droplet as a Tool 45 -- 3.1 Introduction -- 3.2 Active Droplet Generation -- 3.2.1 Electrical Control -- 3.2.2 Magnetic Control -- 3.2.3 Thermal Control -- 3.2.4 Pneumatic Control -- 3.2.5 Piezoelectric Control -- 3.3 Active Droplet Sorting -- 3.3.1 Electrical Control -- 3.3.2 Magnetic Control -- 3.3.3 Thermal Control |
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3.3.4 Pneumatic Control -- 3.3.5 Acoustic Control -- 3.4 Active Droplet Coalescence -- 3.4.1 Electric Control -- 3.4.2 Magnetic Control -- 3.4.3 Thermal Control -- 3.4.4 Pneumatic Control -- 3.4.5 Acoustic Control -- 3.4.6 Optical Control -- 3.5 Conclusion -- References -- Chapter 4 Droplet Gene Analysis -- Digital PCR 89 -- 4.1 Introduction -- 4.2 Droplet Digital PCR Workflow -- 4.3 Fluorophores for ddPCR Applications -- 4.4 Surfactants -- Droplet Stabilizing Agents -- 4.5 Molecular Adsorption/retention -- 4.6 Template Preparation -- 4.7 Multiplex Assays |
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4.8 Commercial Droplet Digital PCR Systems -- 4.8.1 Bio-Rad QX200 -- 4.8.2 RainDrop Plus -- 4.8.3 Stilla Technologies' Naica -- 4.8.4 Comparison of Commercial Systems -- 4.9 Droplet Digital PCR Applications -- 4.10 Emerging Alternative Techniques -- References -- Chapter 5 Microfluidic Applications in Single-cell Genomic, Transcriptomic and Proteomic Analysis 122 -- 5.1 Introduction -- 5.1.1 Single-cell Analysis in Biology -- 5.1.2 Single-cell Analysis in Cancer -- 5.1.3 Microfluidics Overview -- 5.2 Genomic DNA Applications: SNV and CNV Detection -- 5.2.1 Overview -- 5.2.2 Workflow |
| Note |
5.2.3 Examples of Data and Conclusions. |
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Edited by two leaders, this book has drawn together expertise from around the globe to form a unified, cohesive resource for the droplet microfluidics community. |
| Local Note |
eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America |
| Subject |
Microdroplets.
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Microdroplets. |
| Genre/Form |
Electronic books.
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Electronic books.
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| Added Author |
Li, Wenqin.
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| Other Form: |
Print version: Ren, Carolyn Droplet Microfluidics Cambridge : Royal Society of Chemistry,c2020 9781788017695 |
| ISBN |
1839162864 |
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9781839162862 (electronic book) |
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