| Description |
1 online resource (364 pages). |
| Physical Medium |
polychrome |
| Description |
text file |
| Series |
ISSN
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ISSN.
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| Note |
Description based upon print version of record. |
| Contents |
Intro -- Half Title -- Title -- Copyright -- Preface -- Contents -- Chapter 1 Identification of Cellular Protein-Protein Interactions -- 1.1 Introduction -- 1.2 Mass Spectrometry-based Proteomics for Identification of PPIs -- 1.2.1 Affinity Purification Coupled to Mass Spectrometry (AP-MS) -- 1.2.2 Proximity-labeling MS: BioID and APEX -- 1.2.3 Crosslinking MS -- XL-MS -- 1.3 Display Methods -- 1.3.1 Proteomic Peptide-phage Display -- ProP-PD -- 1.3.2 mRNA Display -- 1.3.3 Yeast Surface Display -- 1.4 Array-based Methods -- 1.4.1 Protein Arrays -- 1.4.2 Peptide Arrays |
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1.5 Fluorescence- and Luminescence-based Methods -- 1.5.1 Förster Resonance Energy Transfer (FRET) -- 1.5.2 Bioluminescence Resonance Energy Transfer (BRET) -- 1.5.3 Luminescence-based Mammalian Interactome Mapping -- LUMIER -- 1.6 Protein Complementation Assays (PCAs) -- 1.6.1 Split DHFR and Split TEV -- 1.6.2 Bimolecular Fluorescence Complementation (BiFC) and Bimolecular Luminescence Complementation (BiLC) Assays -- 1.7 Two-hybrid Methods -- 1.7.1 Yeast Two-hybrid (Y2H) -- 1.7.2 MYTH and MaMTH -- 1.7.3 Mammalian PPI Trap (MAPPIT) -- 1.8 Proximity Ligation Assay (PLA) -- 1.9 Concluding Remarks |
| Bibliography |
References -- Chapter 2 Target-based Screening and Structure-based Design Approaches to Develop Modulators of Protein-Protein Interactions -- 2.1 The Challenge with Targeting Protein-Protein Interactions -- 2.2 Modulation of Protein-Protein Interactions -- 2.3 Classifying Protein-Protein Interactions -- 2.4 Overview of Approaches to Target Protein-Protein Interactions -- 2.5 Phenotypic Screening -- 2.5.1 Phenotypic Screening: Overview, Benefits, and Drawbacks -- 2.5.2 Phenotypic Screening Workflow -- 2.6 Target-based Approaches: Target-based Rational Design and Target-based Screening. |
| Contents |
2.6.1 Target-based Approaches: Overview, Benefits, and Drawbacks -- 2.6.2 Target-based: Rational Design Workflow -- 2.6.3 Target-based Screening Workflow -- 2.7 A Case Study: KIX/MLL -- 2.8 Conclusion and Outlook -- References -- Chapter 3 The Challenge of Prosecuting Intracellular Protein-Protein Interactions: A Multi-modality Examination of Chemical Matter Identification, Biophysical Characterization, and Membrane Permeability -- 3.1 Introduction -- 3.2 Biophysical Characterization of Protein-Ligand Interactions: An Essential Aspect of Discovery and Development |
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3.3 Small Molecules: The Preferred Entity for Drugging Intracellular PPIs -- 3.3.1 DNA-encoded Library Technology (DELT) -- 3.3.2 Targeted Protein Degradation -- 3.4 Macrocyclic Peptides-Right Technology, Right Time? -- 3.4.1 Hit Identification and Application to Intracellular PPIs -- 3.4.2 Permeability as the Central Challenge -- 3.4.3 Strategies for Optimizing Ligandability and Permeability -- 3.4.4 Considerations Beyond Ligandability and Permeability -- 3.5 Antibody Approaches to Intracellular PPIs: Break on Through to the Other Side |
| Note |
3.5.1 Antibody Structural Modification to Achieve Cellular Permeability Properties. |
| Summary |
Illustrated throughout with successful case studies, this book provides a holistic, cutting-edge view of the subject area and is ideal for chemical biologists and medicinal chemists interested in developing PPI inhibitors. |
| Local Note |
eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America |
| Subject |
Protein-protein interactions.
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Protein-protein interactions. |
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Molecule-molecule collisions.
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Molecule-molecule collisions. |
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Contact inhibition (Biology)
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Contact inhibition (Biology) |
| Genre/Form |
Electronic books.
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Electronic books.
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| Other Form: |
Print version: Tavassoli, Ali Inhibitors of ProteinProtein Interactions : Small Molecules, Cyclic Peptides, Macrocycles and Antibodies Cambridge : Royal Society of Chemistry,c2020 9781788015691 |
| ISBN |
1839160705 |
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9781839160707 (electronic book) |
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9781839160677 (PDF ebook) |
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1839160675 |
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9781788015691 hardback |
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