| Description |
1 online resource (xxii, 248 pages) : illustrations (some color) |
|
data file |
| Physical Medium |
polychrome |
| Bibliography |
Includes bibliographical references and index. |
| Contents |
Valid analytical molecular biology : the challenge -- Quality in the analytical molecular biology laboratory -- An introduction to method validation -- DNA extraction -- DNA quantification -- PCR : factors affecting reliability and validity -- Quantitative real-time PCR analysis -- Multiplex PCR and whole genome amplification -- Procedures for quality control of RNA samples for use in quantitative reverse transcription PCR -- Microarrays. |
| Summary |
Over the last decade there has been a rapid development of molecular techniques, with an increasing range of instrumentation now available. The development of accompanying reference literature has not kept pace with technological advances and this poses significant challenges to the analyst. Essentials of Nucleic Acid Analysis sets out to guide the analyst through the steps needed to obtain good quality results in DNA analysis. The underlying principles for achieving this goal were formulated by LGC (formerly the Laboratory of the Government Chemist) as the six principles for ensuring valid analytical measurement, which are detailed in the introduction. The reader is also provided with guidelines for method validation and quality control of established and emerging DNA measurement techniques. The authors of each chapter are practitioners of the art of DNA analysis in areas where the quality of the result is critical. Technical details and examples of application of key techniques in nucleic acid analysis are provided while highlighting best practice, available standards and practical advice on improving measurement quality. This book provides an indispensable handbook and premier reference for those working in the widely varying areas and specifically in the fields of food analysis and forensic applications. |
| Access |
Use copy Restrictions unspecified MiAaHDL |
| Reproduction |
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL |
| System Details |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
| Processing Action |
digitized 2010 HathiTrust Digital Library committed to preserve MiAaHDL |
| Access |
Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK). WlAbNL |
| Local Note |
eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America |
| Language |
English. |
| Terms Of Use |
Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL |
| Subject |
Nucleic acids -- Analysis.
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Nucleic acids -- Analysis. |
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Analytical chemistry. |
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Molecular biology. |
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SCIENCE -- Chemistry -- Organic. |
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Science. |
| Indexed Term |
Handbooks |
| Genre/Form |
Electronic books.
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Electronic books.
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| Added Author |
Keer, Jacquie T.
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Birch, Lyndsey.
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| Other Form: |
Print version: Essentials of nucleic acid analysis. Cambridge, UK : Royal Society of Chemistry, ©2008 9780854043675 (DLC) 2008299124 (OCoLC)183916652 |
| ISBN |
9781615833597 (electronic book) |
|
1615833595 (electronic book) |
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9781847558213 (electronic book) |
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1847558216 (electronic book) |
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9780854043675 (hardback) |
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0854043675 (hardback) |
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