| Description |
1 online resource (xii, 480 pages) : illustrations |
| Physical Medium |
polychrome |
| Description |
text file |
| Summary |
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features * Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method. |
| Contents |
Contributors. -- Introduction: The Art of Expression. -- Purpose of This Book. -- Selecting a Suitable Expression System: -- Considerations. -- Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. -- Prokaryotic Expression Systems: -- Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. -- Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. -- Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy. -- Index. |
| Bibliography |
Includes bibliographical references and index. |
| Contents |
Gene expression systems based on bacteriophage T7 RNA polymerase -- Expression vectors employing the trc promoter -- Bacillus expression : a gram-positive model -- araB expression system in Escherichia coli -- Adenoviral vectors for protein expression -- Expression in the methylotrophic yeast Pichia pastoris -- Recombinant protein expression in Pichia methanolica -- Cytomegalovirus promoter for expression in mammalian cells -- Inducible mammalian expression systems -- Protein expression in mammalian cells using Sindbis virus -- Drosophila S2 system for heterologous gene expression -- Baculovirus expression vector system -- Recombinant protein expression in transgenic mice -- Expression of recombinant proteins in the milk of transgenic animals -- Recombinant protein expression in plants. |
| Access |
Use copy Restrictions unspecified MiAaHDL |
| Reproduction |
Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL |
| System Details |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
| Processing Action |
digitized 2010 HathiTrust Digital Library committed to preserve MiAaHDL |
| Local Note |
eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America |
| Subject |
Gene expression.
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Gene expression. |
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Prokaryotes -- Genetics.
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Prokaryotes -- Genetics. |
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Prokaryotes. |
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Insects -- Genetics.
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Insects -- Genetics. |
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Transgenic animals -- Genetics.
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Transgenic animals. |
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Genetics. |
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Eukaryotic cells.
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Eukaryotic cells. |
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Gene Expression Regulation. |
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Gene Expression. |
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Prokaryotic Cells. |
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Animals, Genetically Modified -- genetics. |
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Eukaryotic Cells. |
| Genre/Form |
Electronic books.
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| Added Author |
Fernandez, Joseph M.
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Hoeffler, James P.
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| Other Form: |
Print version: Gene expression systems. San Diego : Academic Press, ©1999 0122538404 9780122538407 (DLC) 98085621 (OCoLC)40462599 |
| ISBN |
9780122538407 |
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0122538404 |
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9780080532356 (electronic book) |
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0080532357 (electronic book) |
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