| Description |
1 online resource (236 pages). |
| Physical Medium |
polychrome |
| Description |
text file |
| Series |
Current and Future Developments in Proteomics Ser.
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Current and Future Developments in Proteomics Ser.
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| Note |
Description based upon print version of record. |
| Contents |
Cover -- Title -- Copyright -- End User License Agreement -- Contents -- Foreword -- Preface -- ACKNOWLEDGEMENTS -- About the Editor -- Dedication -- List of Contributors -- Microbial Biofilm and Drug Resistance: A Proteomic Approach -- Sarika Sharma1 and Sandeep Sharma2,* -- INTRODUCTION -- The Process of Biofilm Formation -- Attachment -- Micro-Colony Formation -- Detachment -- Mechanism of Biofilm Resistance against Antimicrobials -- Limited Penetration of Drugs Due to Glycocalyx -- Enzyme-Mediated Resistance -- State of Metabolism and Rate of Growth in Biofilms -- Genetic Adaptations |
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Efflux Pumps -- Quorum Sensing -- TARGET PROTEINS TO STUDY BIOFILM -- Proteins Involved in Cellular Metabolism -- Transporter Proteins -- Stress-responsive Proteins -- Proteomic Techniques to Study Biofilm -- Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) -- Isotope-coded Affinity Tags (ICAT) -- Isobaric Tags for Relative and Absolute Quantification (iTRAQ) -- Tandem Mass Tags (TMT) -- Isotope-coded Protein Label (ICPL) -- Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) -- Future Prospectus -- CONCLUSION -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS |
| Bibliography |
REFERENCES -- Past, Present, and Future of Gel-Based Microbial Proteomics -- Munna Lal Yadav1,*, Arvind K. Verma1, Preeti Rawat1, Abhishek Parashar1, Divakar Sharma2, Sudarshan Kumar1 and Ashok K. Mohanty1,* -- INTRODUCTION -- PROTEIN SAMPLE PREPARATION FROM MICROBIAL SOURCE -- CLEAN-UP AND QUANTIFICATION OF PROTEINS -- GEL ELECTROPHORESIS OF PROTEINS -- TYPES OF PAGE -- SDS-PAGE (Sodium Dodecyl Sulphate-polyacrylamide Gel Electrophoresis) -- Tricine SDS-PAGE -- Native Page -- SDD-AGE (Semi Denaturing Detergent-agarose Gel Electrophoresis) -- 2D-PAGE (Two-dimensional Gel Electrophoresis). |
| Contents |
Isoelectric Focusing (First Dimension) -- SDS-PAGE (Second Dimension) -- 2D-DIGE (TWO DIMENSIONAL DIFFERENCE IN-GEL ELECTROPHORESIS) -- ANALYSIS OF 2D GEL AND DIGE GEL BY DIFFERENT SOFTWARE -- 2D Gel Analysis using Image Master 2D Platinum -- Evaluation of DIGE Gels using DeCyder -- COMPARISON BETWEEN IEF, SDS-PAGE, 2D-PAGE, AND DIGE -- MICROBIAL PROTEOMICS IN DAIRY PROCESSING -- FUTURE PERSPECTIVES OF GEL BASED MICROBIAL PROTEOMICS -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES |
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Liquid Chromatography-Mass Spectrometry (LCMS): An Advanced Tool for the Microbial Proteomics Analysis -- Pranav Kumar Prabhakar* -- INTRODUCTION -- MASS SPECTROSCOPY (MS) -- Shotgun MS-Based Proteomics -- Shotgun MS-Based Quantitative Proteomics -- EXPERIMENTAL PROCEDURE -- i. Sample Preparation -- ii. Mass Spectrometry -- DATA ACCOMPLISHMENT -- PROTEIN IDENTIFICATION -- USE OF MALDI-TOF IN MICROBIAL IDENTIFICATION AND ANTIMICROBIAL RESISTANCE -- CONCLUSION -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES |
| Note |
Functional Annotation and Enrichment of Microbial Proteins Using Systems Biology: Tools and Applications. |
| Local Note |
eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America |
| Subject |
Microbial proteins.
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Microbial proteins. |
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Proteomics.
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Proteomics. |
| Genre/Form |
Electronic books.
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| Added Author |
Sharma, Divakar.
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| Other Form: |
Print version: Sharma, Divakar Microbial Proteomics: Development in Technologies and Applications Singapore : Bentham Science Publishers,c2020 9789811491429 |
| ISBN |
9811491410 |
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9789811491412 (electronic book) |
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