| Description |
1 online resource (362 pages). |
| Physical Medium |
polychrome |
| Description |
text file |
| Series |
ISSN
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ISSN.
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| Note |
Description based upon print version of record. |
| Contents |
Intro -- Title -- Copyright -- Preface -- Contents -- Chapter 1 Modern Mass Spectrometry and Advanced Fragmentation Methods -- 1.1 Introduction -- 1.2 Ionisation Methods -- 1.3 Figures of Merit for Mass Spectrometry -- 1.4 Common Mass Analysers -- 1.5 New Approaches: Native MS and Ion Mobility -- 1.6 Limitations to Precursor Mass Determination Alone and the Need for Advanced Fragmentation Methods -- 1.7 Conclusion -- Acknowledgements -- References -- Chapter 2 Mechanism and Implementation of Electron Capture and Electron Transfer Dissociation -- 2.1 Introduction |
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2.2 Proposed ECD and ETD Mechanisms -- 2.2.1 Cornell Mechanism -- 2.2.2 Utah-Washington Mechanism -- 2.2.3 Other Mechanisms -- 2.2.4 ETD -- 2.2.5 Side Reactions -- 2.2.6 Electron-based Dissociation of Anions -- 2.3 Implementation of ECD and ETD -- 2.3.1 Directly and Indirectly Heated Cathodes in FTICR -- 2.3.2 Implementation in Radiofrequency Ion Traps -- 2.3.3 Atmospheric Pressure-ECD -- 2.3.4 Add-on Reaction Modules -- 2.3.5 ETD Implementation -- 2.4 Conclusion -- Acknowledgements -- References -- Chapter 3 ECD/ETD for Sequencing of Peptides and Denatured Proteins -- 3.1 Introduction |
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3.2 Instrument Requirements for ECD and ETD: A Proteomics Perspective -- 3.3 Practical Considerations on ECD/ETD Reaction Products -- 3.4 Role of Precursor Mass, Charge State and Residue Selectivity -- 3.5 Residual Higher-order Structure in Polypeptide Cations and the Need for Supplemental Activation -- 3.6 ECD and ETD in Bottom-up Protein Analysis -- 3.7 Characterization of Larger Polypeptides via ECD and ETD: Top-down and Middle-down MS -- 3.8 Characterization of Post-translational Modifications -- 3.9 Outlook and Future Perspectives -- Acknowledgements -- References |
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Chapter 4 Structural Studies using Electron-based Fragmentation Methods and Chemical Labelling of Proteins -- 4.1 Introduction -- 4.2 State of the Art and Current Challenges of HDX-MS -- 4.3 Increasing Spatial Resolution in HDX-MS -- 4.4 Use of ExD Methods to Achieve Single-residue Resolution in HDX-MS -- 4.5 Native Top-down ECD and ETD Fragmentation -- 4.6 Top-down HDX with High Spatial Resolution -- 4.7 Avoiding Gas-phase Hydrogen Scrambling in Negative Ion Mode Tandem MS -- 4.8 UVPD as a New Modality for Electron-driven Fragmentation -- 4.9 Cleavable Crosslinkers -- 4.10 Conclusions |
| Bibliography |
References -- Chapter 5 Investigating 3D Structures of Native Proteins and Complexes through Electron-based Dissociation -- 5.1 Introduction -- 5.2 Applying ExD Combined with Crosslinking and Hydrogen-Deuterium Exchange of Intact Proteins -- 5.2.1 Introduction to Hydrogen-Deuterium Exchange -- 5.2.2 Hydrogen-Deuterium Exchange on Intact Proteins with ExD Fragmentation -- 5.2.3 Introduction to Chemical Crosslinking for Structure Determination -- 5.2.4 Potential Advantages of CXL Analysis on MS of Intact Proteins -- 5.3 ESI-ExD of Native Proteins and Protein Complexes. |
| Note |
5.3.1 ECD and ETD of Intact Proteins and Protein Complexes. |
| Summary |
This book is a high-level introduction, as well as a reference work for experienced users, to ECD, ETD, EDD, NETD, UVPD, SID, and other advanced fragmentation methods. |
| Local Note |
eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America |
| Subject |
Fragmentation reactions.
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Fragmentation reactions. |
| Genre/Form |
Electronic books.
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Electronic books.
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| Added Author |
Lermyte, Frederik.
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| Other Form: |
Print version: Lermyte, Frederik Advanced Fragmentation Methods in Biomolecular Mass Spectrometry : Probing Primary and Higher Order Structure with Electrons, Photons and Surfaces Cambridge : Royal Society of Chemistry,c2020 9781839161049 |
| ISBN |
1839161108 |
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9781839161100 (electronic book) |
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