| Description |
1 online resource (415 pages). |
| Physical Medium |
polychrome |
| Description |
text file |
| Series |
ISSN
|
|
ISSN.
|
| Note |
Description based upon print version of record. |
| Contents |
Intro -- Halftitle -- Detection Science Series -- Title -- Copyright -- Preface -- Contents -- Chapter 1 Protein-Protein Interaction Interfaces and their Functional Implications -- 1.1 Introduction -- 1.2 Categorizing Protein-Protein Interactions -- 1.3 Biophysical Principles Governing the Specificity of Protein-Protein Interactions -- 1.4 Amino Acid Contributions Towards Association and Dissociation Rate Constants -- 1.5 Structural Changes Associated with Formation of Protein-Protein Complexes -- 1.6 General Characteristics of PPI Interfaces -- 1.7 The Role of Interface Water in Binding |
|
1.8 The Core-Rim Model of Protein-binding Interfaces -- 1.9 Binding Interfaces Viewed as Connected Modules -- 1.10 Enthalpy-Entropy Compensation in PPIs -- 1.11 Protein-binding Sites are Imprinted in the Unbound Structure -- 1.12 Conservation of Interface Residues -- 1.13 Concluding Remarks -- Acknowledgements -- References -- Chapter 2 Protein-Protein Interaction Networks in Human Disease -- 2.1 Introduction -- 2.2 Methods Commonly Employed in Disease PPI Network Analysis -- 2.2.1 Yeast-two-hybrid -- 2.2.2 Protein Microarray -- 2.2.3 Affinity-purification Coupled to Mass Spectrometry |
|
2.2.4 Proximity Labelling Methods -- 2.3 Role of PPI Networks in Human Diseases -- 2.3.1 Cancer -- 2.3.2 Parkinson's Disease -- 2.3.3 Cardiomyopathies -- 2.3.4 Diabetes -- 2.3.5 Autoimmune Diseases -- 2.3.6 Microbial Diseases -- 2.4 Challenges in Working with PPI Networks and Overcoming these Challenges -- 2.5 Conclusion -- Funding -- References -- Chapter 3 High Throughput Screening Methods for PPI Inhibitor Discovery -- 3.1 Introduction -- 3.2 HTS Methods for PPI Inhibitor Discovery -- 3.2.1 Overview -- 3.2.2 Methods to Detect Direct Interactions -- 3.2.3 Proximity-based Energy Transfer Methods |
|
3.2.4 Protein Fragment Complementation Assay (PCA) Methods -- 3.2.5 Phenotype-based Methods: High Content Screening -- 3.3 Validation Methods for PPI Inhibitor Discovery -- 3.3.1 Biosensor Technologies: Surface Plasmon Resonance and Biolayer Interferometry -- 3.3.2 Affinity Capture Techniques: Co-immunoprecipitation and Pull-down -- 3.3.3 Ligand-observed NMR and Protein-observed NMR -- 3.3.4 X-ray Crystallography -- 3.3.5 Isothermal Titration Calorimetry -- 3.3.6 Differential Scanning Fluorometry (Protein Thermal Shift Assay) -- 3.3.7 Cellular Thermal Shift Assay |
|
3.3.8 Microscale Thermophoresis -- 3.3.9 Proximity Ligation Assay -- 3.4 Optimization of Identified PPI Inhibitors -- 3.5 Conclusion -- Acknowledgements -- References -- Chapter 4 Computational Structural Modeling to Discover PPI Modulators -- 4.1 Introduction -- 4.2 Protein Structure Availability and Modeling -- 4.2.1 Swiss-model -- 4.2.2 I-TASSER -- 4.3 Identification of the PPI Interface and Small Molecule Binding Site -- 4.3.1 Protein-Protein Docking Tools and Software -- 4.3.2 Binding Pocket Identification -- 4.4 Virtual Screening for Small Molecule PPI Modulators -- 4.4.1 AutoDock Vina |
| Note |
4.4.2 USCF DOCK. |
| Summary |
Protein-Protein Interaction Regulators surveys the latest advances in the structural understanding of PPIs as well as recent developments in modulator discovery. |
| Local Note |
eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America |
| Subject |
Protein-protein interactions.
|
|
Protein-protein interactions. |
|
Drug development.
|
|
Drug development. |
| Genre/Form |
Electronic books.
|
|
Electronic books.
|
| Added Author |
Roy, Siddhartha, 1954-
|
|
Fu, Haian.
|
| Other Form: |
Print version: Roy, Siddhartha ProteinProtein Interaction Regulators Cambridge : Royal Society of Chemistry,c2020 9781788011877 |
| ISBN |
1839160500 |
|
9781839160509 (electronic book) |
|
