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Title Protein phosphorylation : a practical approach / edited by D.G. Hardie.

Publication Info. London ; New York : Oxford University Press, 1999.

Item Status

Edition 2nd ed.
Description 1 online resource (xxviii, 431 pages) : illustrations.
Physical Medium polychrome
Description text file
Series The practical approach series ; 211
Practical approach series ; 211.
Bibliography Includes bibliographical references and index.
Contents Study of protein phosphorylation in intact cells -- Analysis of signal transduction pathways using protein kinase inhibitors and activators -- Analysis of signal transduction pathways using protein-serin/threonine phosphatase inhibitors -- Analysis of signal transduction pathways using molecular biological manipulations -- Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates -- Phosphorylation site analysis by mass spectrometry -- Assay and purification of protein (serine/threonine) phosphatases -- Identification of substrates for protein-tyrosine phosphateases -- Assay and purification of protein-serine/threonine kinases -- Assay of protein kinases and phosphatases using specific peptide substrates -- Cloning and expression of cDNAs encoding protein kinase subunits -- Interaction cloning of protein kinase partners -- Identifying protein kinase substrates by expression screening with solid-phase phosphorylation -- Analysis of protein kinase interactions using the two-hybrid method -- Analysis of protein kinase interactions using biomolecular interaction analysis -- Analysis of protein kinase specificity using oriented peptide libraries -- Crystallization of protein kinases and phosphatases.
Local Note eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America
Subject Phosphoproteins -- Laboratory manuals.
Phosphoproteins.
Genre/Form Laboratory manuals.
Subject Phosphorylation -- Laboratory manuals.
Phosphorylation.
Protein kinases -- Laboratory manuals.
Protein kinases.
Phosphoprotein phosphatases -- Laboratory manuals.
Phosphoprotein phosphatases.
Phosphorylation.
Protein Kinases.
Genre/Form Electronic books.
Laboratory manuals.
Added Author Hardie, D. G. (D. Grahame)
Other Form: Print version: Protein phosphorylation. 2nd ed. London ; New York : Oxford University Press, 1999 0199637296 0199637288 (DLC) 99032319 (OCoLC)41380595
ISBN 0585483930 (electronic book)
9780585483931 (electronic book)