Skip to content
You are not logged in |Login  
     
Limit search to available items
Record:   Prev Next
Resources
More Information
Bestseller
BestsellerE-book

Title Arabidopsis : a practical approach / edited by Zoe A. Wilson.

Publication Info. Oxford ; New York : Oxford University Press, 2000.

Item Status

Description 1 online resource (xix, 275 pages) : illustrations.
Physical Medium polychrome
Description text file
Series The practical approach series ; PAS/223
Practical approach series ; 223.
Bibliography Includes bibliographical references and index.
Contents 1. Growth, maintenance, and use of Arabidopsis genetic resources / Mary Anderson, Fiona Wilson 1 -- 1. What is Arabidopsis? 1 -- 2. What makes Arabidopsis such an attractive experimental model? 3 -- 3. Arabidopsis genetic resource centres 4 -- Arabidopsis genetic resources 4 -- Accessing Arabidopsis resources 5 -- 4. Mutants of Arabidopsis 5 -- Single gene mutation lines 5 -- Resources for the identification/investigation of novel genes 8 -- Mapping tools 10 -- 5. Considerations of available resources for identifying novel genes 11 -- Forward genetics 12 -- Reverse genetics 14 -- 6. Growing Arabidopsis 15 -- How to maintain clean growth conditions 15 -- Growing Arabidopsis in the glasshouse 16 -- Chemical control of pests and diseases 19 -- 7. Seed storage 21 -- 8. Growing Arabidopsis with specific growth requirements 23 -- 9. Sterile culture of Arabidopsis 24 -- 2. Preservation and handling of stock centre clones / Randy Scholl, Keith Davis, Doreen Ware 29 -- 2. Missions of a plant DNA resource centre 29 -- 3. Preservation of stocks 30 -- Plasmids with small DNA inserts 30 -- Cosmids 32 -- Phage and phage libraries 33 -- Yeast artificial chromosome (YAC) libraries 33 -- Pools of YAC library cells for PCR screening 37 -- Distribution of YAC libraries arrayed on nylon filters 38 -- Other large-insert libraries 38 -- Yeast expression analysis--'two-hybrid' libraries and complementation testing 42 -- 4. Verification of stock identity and purity 42 -- 5. Pooled DNA from T-DNA lines for PCR screening 44 -- 6. Organization of stock information 48 -- Collecting, maintaining, and disseminating stock data 48 -- Organizing and distributing patron data 48 -- 7. Future 48 -- 3. Genetic mapping using recombinant inbred lines / Clare Lister, Mary Anderson, Caroline Dean 51 -- 2. Preparation and digestion of A. thaliana genomic DNA 54 -- Preparation of genomic DNA 56 -- Identifying an RFLP 60 -- Southern blotting and hybridization 61 -- 3. Polymorphic markers 62 -- RFLP markers 62 -- PCR-based markers 66 -- Phenotypic and biochemical markers 68 -- 4. Calculating map positions 69 -- Mapping programs 69 -- NASC mapping service 70 -- 5. Integration of a mutation into a molecular map 72 -- 4. Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis / Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson 77 -- 1. Generation of mutants and their importance for developmental biology 77 -- 2. Mapping and segregation analysis 78 -- Mapping of mutations 78 -- Influence of environment on phenotype 82 -- 3. Microscopy 82 -- Fresh material characterization 82 -- Fixed material characterization 84 -- 4. Analysis of plant gene expression 89 -- RNA isolation 89 -- Northern analysis 91 -- In situ hybridizations 92 -- 5. Classical and molecular cytogenetics of Arabidopsis / G.H. Jones, J.S. Heslop-Harrison 105 -- 2. Mitotic chromosome analysis by light microscopy 108 -- 3. In situ hybridization to mitotic chromosome preparations 112 -- Photography of in situ hybridizations 117 -- 4. Meiotic chromosome analysis by light microscopy 117 -- 5. Meiotic chromosome analysis by electron microscopy 121 -- 6. Tissue culture, transformation, and transient gene expression in Arabidopsis / Keith Lindsey, Wenbin Wei 125 -- 2. Stable transformation by Agrobacterium tumefaciens 128 -- 3. Transient gene expression in Arabidopsis protoplasts 131 -- Reporter gene enzyme assays 134 -- 7. Transposon and T-DNA mutagenesis / Mark G.M. Aarts, Csaba Koncz, Andy Pereira 143 -- 2. Transposon tagging 143 -- Endogenous transposable elements 143 -- Transposon tagging systems in Arabidopsis 144 -- Which system to use? 149 -- Genetic and molecular analysis of a putatively transposon tagged mutant 151 -- Further applications of transposon tagging 156 -- 3.2 T-DNA tagging 158 -- Use of T-DNA as insertional mutagen 158 -- Random tagging 158 -- Available populations of T-DNA transformants 159 -- Promoter/enhancer trapping 160 -- Analysis of T-DNA mutants and cloning a tagged gene 161 -- Further applications of T-DNA tagging 166 -- 8. Map-based cloning in Arabidopsis / Joanna Putterill, George Coupland 171 -- 2. Locating the mutation of interest relative to DNA markers 172 -- Determining an approximate map position 172 -- Identifying a short genetic interval containing the mutation as a prelude to isolating the gene 175 -- 3. Placing the gene on the physical map 177 -- Chromosome landing 177 -- Chromosome walking with YAC clones 178 -- 4. Identification of the gene 189 -- Location of the gene by molecular complementation 189 -- Determining the structure of the gene 194 -- 5. Perspectives 194 -- 9. Physical mapping: YACs, BACs, cosmids, and nucleotide sequences / Ian Bancroft 199 -- 2. Genome mapping with YAC clones 199 -- 3. Genome mapping with BAC and P1 clones 202 -- Communal resources 202 -- Construction of BAC libraries 208 -- Genome mapping with BACs 211 -- 4. High resolution mapping with cosmids 216 -- Approaches to mapping with cosmids 216 -- Construction of cosmid libraries 216 -- 5. Nucleotide sequences--the ultimate mapping tool 221 -- EST sequencing project 221 -- Genome sequencing project 221 -- Sequence-based mapping 222 -- 10. Web-based bioinformatic tools for Arabidopsis researchers / Seung Y. Rhee, David J. Flanders 225 -- What is bioinformatics 225 -- Sources of Arabidopsis bioinformatic data 226 -- 2. Basic tools for the Internet 227 -- Web basics 227 -- Getting onto the Web 228 -- Using your browser 229 -- Browser tips and errors 232 -- Privacy issues 235 -- 3. Scenarios of bioinformatic use in Arabidopsis research 235 -- 4. Gene information resources 237 -- General gene information 237 -- Arabidopsis gene information 237 -- Plant gene information 238 -- Genetic maps 239 -- Physical maps 240 -- 6. Sequencing 242 -- Arabidopsis genome initiative (AGI) 243 -- Annotation of sequences by AGI 243 -- Caveats in annotation 244 -- Sequence contigs from AtDB 245 -- 7. Sequence analysis tools 245 -- BLAST 246 -- FASTA 250 -- EST databases 252 -- Gene identification programs 253 -- Gene family analyses 255 -- Motif analyses 255 -- Protein structures 258 -- Comprehensive sequence analysis tools 259 -- 8. Current issues and future directions in bioinformatics 260 -- Some important bioinformatic issues 260 -- Bioinformatic tools currently under development for Arabidopsis research.
Local Note eBooks on EBSCOhost EBSCO eBook Subscription Academic Collection - North America
Subject Arabidopsis -- Laboratory manuals.
Arabidopsis.
Genre/Form Laboratory manuals.
Subject Arabidopsis -- Molecular aspects -- Laboratory manuals.
Arabidopsis -- genetics.
Plants, Genetically Modified -- genetics.
Genre/Form Laboratory Manual.
Electronic books.
Laboratory manuals.
Added Author Wilson, Zoe A.
Other Form: Print version: Arabidopsis. Oxford ; New York : Oxford University Press, 2000 019963565X 0199635641 (DLC) 99045851 (OCoLC)42295871
ISBN 0585483795 (electronic book)
9780585483795 (electronic book)