LEADER 00000cam a2200709Ia 4500 001 ocm53956459 003 OCoLC 005 20160527040455.9 006 m o d 007 cr cnu---unuuu 008 031227s2000 enka ob 001 0 eng d 019 814379150|a824546098|a880329572 020 0585483396|q(electronic book) 020 9780585483399|q(electronic book) 020 128037568X 020 9781280375682 035 (OCoLC)53956459|z(OCoLC)814379150|z(OCoLC)824546098 |z(OCoLC)880329572 040 N$T|beng|epn|cN$T|dOCLCQ|dYDXCP|dOCLCQ|dOCLCF|dNLGGC |dOCLCO|dOCLCQ|dSLY|dIDEBK|dOCLCQ 049 RIDW 050 4 QP551|b.P6965 2000eb 070 QP551|b.P6965 2000 072 7 SCI|x007000|2bisacsh 072 7 MBGL|2bicssc 082 04 572/.636|222 084 Q510. 3|2clc 090 QP551|b.P6965 2000eb 245 00 Protein localization by fluorescence microscopy :|ba practical approach /|cedited by Victoria J. Allan. 264 1 Oxford ;|aNew York :|bOxford University Press,|c[2000] 264 4 |c©2000 300 1 online resource (xxiv, 231 pages) :|billustrations (some color). 336 text|btxt|2rdacontent 337 computer|bc|2rdamedia 338 online resource|bcr|2rdacarrier 340 |gpolychrome|2rdacc 347 text file|2rdaft 490 1 The practical approach series ;|v218 504 Includes bibliographical references and index. 520 There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user. 588 0 Print version record. 590 eBooks on EBSCOhost|bEBSCO eBook Subscription Academic Collection - North America 650 0 Proteins|xAnalysis.|0https://id.loc.gov/authorities/ subjects/sh85107668 650 0 Fluorescence microscopy.|0https://id.loc.gov/authorities/ subjects/sh85049410 650 0 Green fluorescent protein.|0https://id.loc.gov/authorities /subjects/sh97006933 650 0 Immunofluorescence.|0https://id.loc.gov/authorities/ subjects/sh85064559 650 2 Sequence Analysis, Protein.|0https://id.nlm.nih.gov/mesh/ D020539 650 2 Microscopy, Fluorescence.|0https://id.nlm.nih.gov/mesh/ D008856 650 2 Luminescent Proteins.|0https://id.nlm.nih.gov/mesh/D008164 650 7 Proteins|xAnalysis.|2fast|0https://id.worldcat.org/fast/ 1079714 650 7 Fluorescence microscopy.|2fast|0https://id.worldcat.org/ fast/928103 650 7 Green fluorescent protein.|2fast|0https://id.worldcat.org/ fast/947607 650 7 Immunofluorescence.|2fast|0https://id.worldcat.org/fast/ 967946 655 4 Electronic books. 700 1 Allan, Victoria J.|0https://id.loc.gov/authorities/names/ n99048704 776 08 |iPrint version:|tProtein localization by fluorescence microscopy.|dOxford ; New York : Oxford University Press, ©2000|z0199637407|z0199637415|w(DLC) 99037444 |w(OCoLC)41834974 830 0 Practical approach series ;|0https://id.loc.gov/ authorities/names/n84709852|v218. 856 40 |uhttps://rider.idm.oclc.org/login?url=http:// search.ebscohost.com/login.aspx?direct=true&scope=site& db=nlebk&AN=98426|zOnline eBook. Access restricted to current Rider University students, faculty, and staff. 856 42 |3Instructions for reading/downloading this eBook|uhttp:// guides.rider.edu/ebooks/ebsco 901 MARCIVE 20231220 948 |d20160615|cEBSCO|tebscoebooksacademic|lridw 994 92|bRID