LEADER 00000cam a2200649 i 4500 001 ocn951217543 003 OCoLC 005 20230113054233.0 006 m o d 007 cr cnu|||unuuu 008 160606s2016 sz a ob 001 0 eng d 015 GBB8L8273|2bnb 016 7 019129488|2Uk 019 951594257 020 9783319313504|q(electronic book) 020 3319313509|q(electronic book) 020 |z9783319313481|q(print) 020 |z3319313487|q(print) 024 7 10.1007/978-3-319-31350-4|2doi 035 (OCoLC)951217543|z(OCoLC)951594257 037 com.springer.onix.9783319313504|bSpringer Nature 040 N$T|beng|erda|epn|cN$T|dYDXCP|dIDEBK|dGW5XE|dN$T|dAZU |dEBLCP|dOCLCF|dCOO|dKSU|dOHI|dJG0|dIAD|dJBG|dICW|dZ5A |dILO|dICN|dOCLCQ|dESU|dIOG|dU3W|dREB|dWYU|dOCLCO|dOCLCA |dMERER|dOCLCO|dUKMGB|dAUD|dAU@|dOCLCO|dOCLCQ|dUKAHL |dOCLCQ|dOCLCA|dAJS|dOCLCO|dOCLCQ 049 RIDW 050 4 QP625.N89 072 7 SCI|x007000|2bisacsh 082 04 572.8/633|223 090 QP625.N89 245 00 Field guidelines for genetic experimental designs in high- throughput sequencing /|cAna M. Aransay, José Luis Lavín Trueba, editors. 264 1 Switzerland :|bSpringer,|c2016. 300 1 online resource (xi, 399 pages) :|billustrations (some color) 336 text|btxt|2rdacontent 337 computer|bc|2rdamedia 338 online resource|bcr|2rdacarrier 340 |gpolychrome|2rdacc 347 text file|2rdaft 504 Includes bibliographical references at the end of each chapter and index. 505 0 The High Throughput Sequencing technologies triple-W discussion: Why to use HTS, What is the optimal HTS method to use, Which data analysis workflow to follow -- Whole genome sequencing recommendations -- Counseling for targeted DNA region re-sequencing -- Transcriptome profiling strategies -- Differential mRNA Alternative Splicing -- microRNA discovery and expression analysis in animals -- Analysis of Long Non-coding RNAs in RNA-seq data -- Ribosome profiling -- Genome-wide analysis of DNA methylation patterns by high-throughput sequencing -- Characterization of DNA-protein interactions: Design and analysis of ChIP-seq experiments -- PAR-CLIP: A genomic technique to dissect RNA-protein interactions -- Metagenomic design and sequencing -- A hitchhiker's guide to Metatranscriptomics -- Eukaryotic Single-Cell mRNA Sequencing -- Eukaryotic Single-Cell DNA Sequencing -- Submitting data to a public repository, the final step of a successful HTS experiment. 520 High throughput sequencing (HTS) technologies have conquered the genomics and epigenomics worlds. The applications of HTS methods are wide, and can be used to sequence everything from whole or partial genomes, transcriptomes, non-coding RNAs, ribosome profiling, to single-cell sequencing. Having such diversity of alternatives, there is a demand for information by research scientists without experience in HTS that need to choose the most suitable methodology or combination of platforms and to define their experimental designs to achieve their specific objectives. Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing aims to collect in a single volume all aspects that should be taken into account when HTS technologies are being incorporated into a research project and the reasons behind them. Moreover, examples of several successful strategies will be analyzed to make the point of the crucial features. This book will be of use to all scientist that are unfamiliar with HTS and want to incorporate such technologies to their research. 588 0 Online resource; title from PDF title page (SpringerLink, viewed June 15, 2016). 590 eBooks on EBSCOhost|bEBSCO eBook Subscription Academic Collection - North America 650 0 Nucleotide sequence.|0https://id.loc.gov/authorities/ subjects/sh85093159 650 0 Combinatorial chemistry.|0https://id.loc.gov/authorities/ subjects/sh96005121 650 7 Nucleotide sequence.|2fast|0https://id.worldcat.org/fast/ 1041106 650 7 Combinatorial chemistry.|2fast|0https://id.worldcat.org/ fast/868966 650 7 Life sciences: general issues.|2bicssc 650 7 Medical genetics.|2bicssc 650 7 SCIENCE|xLife Sciences|xBiochemistry.|2bisacsh 700 1 Aransay, Ana M.,|0https://id.loc.gov/authorities/names/ no2014146633|eeditor. 700 1 Lavín Trueba, José Luis,|0https://id.loc.gov/authorities/ names/n2016188363|eeditor. 776 08 |iPrint version:|tField Guidelines for Genetic Experimental Designs in High-Throughput Sequencing.|dCham : Springer International Publishing, ©2016|z9783319313481 856 40 |uhttps://rider.idm.oclc.org/login?url=https:// search.ebscohost.com/login.aspx?direct=true&scope=site& db=nlebk&AN=1188309|zOnline ebook via EBSCO. Access restricted to current Rider University students, faculty, and staff. 856 42 |3Instructions for reading/downloading the EBSCO version of this ebook|uhttp://guides.rider.edu/ebooks/ebsco 901 MARCIVE 20231220 948 |d20230203|cEBSCO|tEBSCOebooksacademic NEW 6073 Quarterly |lridw 994 92|bRID